Gene description for Hspa8
Gene name heat shock protein 8
Gene symbol Hspa8
Other names/aliases 2410008N15Rik
Hsc70
Hsc71
Hsc73
Hsp73
Hspa10
Species Mus musculus
 Experiment description of studies that identified Hspa8 in Insulinoma cells

1
Experiment ID1279
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 1
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%

2
Experiment ID1280
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 2
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%

3
Experiment ID1281
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 3
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%

4
Experiment ID1282
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%

5
Experiment ID1283
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%

6
Experiment ID1284
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 22345510   
OrganismRattus norvegicus
Experiment descriptionCharacterization of membrane-shed microvesicles from cytokine-stimulated .-cells using proteomics strategies.
AuthorsPalmisano G, Jensen SS, Le Bihan MC, Lainé, McGuire JN, Pociot F, Larsen MR.
Journal name Mol Cell Proteomics.
Publication year2012
SampleInsulinoma cells
Sample nameNHI 6F Tu28, cytokine-stimulated Replicate 3
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120151: EV-METRIC:14%, 0%

7
Experiment ID1019
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 21734072   
OrganismMus musculus
Experiment descriptionInsulinoma-released exosomes or microparticles are immunostimulatory and can activate autoreactive T cells spontaneously developed in nonobese diabetic mice.
AuthorsSheng H, Hassanali S, Nugent C, Wen L, Hamilton-Williams E, Dias P, Dai YD.
Journal name J Immunol.
Publication year2011
SampleInsulinoma cells
Sample nameMIN6
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV110115: EV-METRIC:11%

8
Experiment ID1020
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 21734072   
OrganismMus musculus
Experiment descriptionInsulinoma-released exosomes or microparticles are immunostimulatory and can activate autoreactive T cells spontaneously developed in nonobese diabetic mice.
AuthorsSheng H, Hassanali S, Nugent C, Wen L, Hamilton-Williams E, Dias P, Dai YD.
Journal name J Immunol.
Publication year2011
SampleInsulinoma cells
Sample nameNIT-1
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV110115: EV-METRIC:11%

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